Application of Differential Ion Mobility Mass Spectrometry to Peptide Quantitation Using SelexIONTM Differential Mobility Separation Technology for better selectivity for peptides in complex mixtures on the AB SCIEX Triple QuadTM 5500 LC/MS/MS System
نویسنده
چکیده
Reduced recovery, low sensitivity – The adsorptive properties and/or polarity of peptides can compromise recovery, and interferences from biological matrices can negatively impact sensitivity and selectivity. Limited quantitation range – Poor MS/MS sensitivity combined with often poor selectivity can compromise the desired lower limits of quantitation (LLOQ). Limited MRM selectivity – MRM approaches and efficient UHPLC separations may not provide adequate signal-to-noise ratios at LLOQ due to isobaric interferences or high baseline noise.
منابع مشابه
Using Differential Mobility Spectrometry to Separate and Localize Sites of Post-Translational Modifications on Peptides
Characterization of the post-translational modification sites (PTMs) on proteins and peptides is a challenging task. Mass spectrometry can provide a good solution for characterization because of the high quality MS/MS spectra that can be obtained for identification and site localization. However, isomeric peptides with PTMs on different residues of the same peptide sequence can be hard to disti...
متن کاملRapid Quantitation of Substance P in Plasma Using Differential Mobility Spectrometry and Micro flow Liquid Chromatography An exceptionally selective and sensitive strategy for high throughput peptide quantification is presented by coupling DMS (Differential Mobility Spectrometry) technology with μLC/MS/MS analysis
• Impaired sensitivity in complex matrices – Very low-level peptide detection (sub-pg/mL) can be suppressed by high background and competing ions in biological samples. • Lack of throughput – Complex biological matrices hamper data resolution and require sophisticated sample preparation and/or advanced instrumentation. • Limited quantitation range – Poor MS/MS sensitivity combined with often po...
متن کاملMiniaturized ultra high field asymmetric waveform ion mobility spectrometry combined with mass spectrometry for peptide analysis.
Miniaturized ultra high field asymmetric waveform ion mobility spectrometry (ultra-FAIMS) combined with mass spectrometry (MS) has been applied to the analysis of standard and tryptic peptides, derived from α-1-acid glycoprotein, using electrospray and nanoelectrospray ion sources. Singly and multiply charged peptide ions were separated in the gas phase using ultra-FAIMS and detected by ion tra...
متن کاملAutomated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS
Automated sample preparation enables highly reproducible quantitative analyses while meeting the increased efficiency needs often required for sample processing in laboratories. This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/ MS and applied to the monitoring of multiple classes of p...
متن کامل